What are the Areas of servers to check for the Slowness of Application Hosted in W2K SP4 server?

Question by Ravikiran K: What are the Areas of servers to check for the Slowness of Application Hosted in W2K SP4 server?
Hi, Any one of you could you please help me out, what are the areas of the server we can check and what way if an application hosted in W2K SP4 server is creating problem with slowness of the applicatin performance and the application is at a time is used by not more than 250 users and all the users are using considerable band width.

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